| The culture supernatants were analysed and butyric acid identified as the potentially toxic material. |
| The culture supernatants, though produced cytopathic effect in various tissue cultures, failed to have any deleterious effect on the worms. |
| Qualitatively, no difference was observed in the cytotoxicity of unheated, heated and ultra filtered culture supernatants of isolates. |
| After various time intervals the supernatants were removed, cell samples were fixed with paraformaldehyde solution for 30 minutes at room temperature. |
| Agglutination reaction was carried out using the reagents with the stool supernatants. |
| The suspensions were spun at 1000 g for 30 minutes and the supernatants were filtered and lyophilized. |
