The cooled, clear digest was diluted to 20 ml with distilled water, filtered, and aliquots were taken for analyses. |
Each sample was then divided into two aliquots which were subsequently treated separately as described below. |
Sample digestion for Hg analysis was conducted separately, using 5 g aliquots of homogenized samples. |
Standard BAL was then performed using three aliquots of 50-ml sterile isotonic saline. |
Mix the diphenylamine reagent, as above, and split it into two aliquots before adding the acetaldehyde. |
The samples tested, including cultured cells, tissue homogenates, and red blood cells, were divided into two aliquots. |