| After extensive washing of the unbound protein with 40 mM imidazole, the GHS protein was eluted with 250 mM imidazole. |
| Concentrated CKs were eluted from the cartridges with ethanol and the eluates were evaporated to dryness. |
| The column was equilibrated with water and eluted in a acetonitrile gradient. |
| The mycotoxin then can be eluted from the column by washing with an organic solvent such as methanol or acetonitrile. |
| The clear supernatant was loaded onto a Ni-chelating column, and bound protein was eluted with 500 mM imidazole. |
| Bound material was then eluted with 0.2 M methyl mannoside in the same buffer. |
