Progeny phage that produced clear plaques in the presence of IPTG and X-gal were analyzed by a differential oligonucleotide hybridization technique, as reported. |
The latter is conjugated to primer oligonucleotides that are pre-annealed to a complementary circular oligonucleotide. |
The quaternary nitrogen atom in lycobetaine plays an important role in the formation of hydrogen bonds between the compound and the oligonucleotide. |
The bands containing labeled oligonucleotide were identified by visual inspection and ultraviolet shadowing. |
Close to the sphere surface, the oligonucleotide interacts with the evanescent field of the WGM, which extends about a wavelength into the buffer solution. |
The claims of this patent cover oligonucleotide compounds comprising a synthetic immunostimulatory dinucleotide motif. |