Luciferase creates light in combination with oxygen, a molecule called luciferin and adenosine triphosphate, a substance found in all living cells. |
Instead of having freely available luciferin, they contain molecules called photoproteins which are, in effect, luciferin proteins that already have oxygen bound to them. |
Mixing of droplets caused enzyme reaction, and luciferin luminesced. |
Nearly all of this light is produced by the oxidization of the molecule luciferin, a reaction catalyzed by the enzyme luciferase. |
Luc activity was assayed quantitatively with a liquid scintillator using the photon-counting mode after the feeding of luciferin as substrate. |
When the pH drops, luciferase changes its shape, allowing luciferin, more specifically tetrapyrrole, to bind. |