The term extinction coefficients describes the absorbance spectra of a single pure substance at known concentrations. |
Upon measuring the absorbance of a labeled peptide at a given angle of incidence, two different dichroic ratios were obtained. |
This signal at extended wavelengths outside of absorbance bands is characteristic of the dispersive part of differential light scattering. |
The absorbance was measured with a microtiter plate reader at a test wavelength of 405 nm. |
The isotropic emission spectra were normalized to the corresponding absorbance at the excitation wavelength. |
The absorbance was always kept below 0.05 to avoid self-absorption of the fluorescence. |