| Cells were perfused at room temperature in a modified Tyrode's solution consisting of 150 mM NaCl, 5 mM KCl, 2 mM CaCl 2, 1 mM MgCl 2, 10 mM Hepes, 30 mM glucose. |
| Replacement of NaCl for isosmotic sorbitol produced a slight depolarization of the membrane. |
| Alternatively, if hypertonic NaCl as not immediately available, hypertonic mannitol can be administered to accomplish this goal. |
| For example this would give us the formula NaCl for sodium chloride. |
| The resulting solution was applied to a gel filtration column and the eluent applied to an anion-exchange column and eluted using a 0-500-mM NaCl gradient. |
| It has a lower eutectic point than NaCl and can work at lower temperatures. |
