“The tissue was sandwiched between a positively charged glass slide and a siliconized coverslip and incubated for 30 min at room temperature.”
“The glass sleeves were inserted into the drilled-out tops of 1.5 ml siliconized polypropylene tubes, and the holder was cemented to the tube top with silicone sealant.”
“The fluid was aspirated after each 60-mL infusion, pooled in a sterile siliconized container, and immediately transported on ice to the laboratory.”
