The second obstacle was the difficulty of transfecting the cell line with plasmids and growing enough virus to be of use as seed stock. |
It can be used in many applications such as these experiments involve transfecting in a gene of interest, and then analyzing the expressed protein. |
The mechanism works by transfecting fish cells with small RNA molecules. |
Adenoviruses can be harnessed to kill cancer cells as well as transfecting them with therapeutic genes thereby further improving efficacy. |