This enabled us to probe the ability of the protein to bind to different phospholipid molecules. |
To prepare multilamellar lipid dispersions, aliquots of CHCl 3 stock solutions of phospholipid and sterol were mixed in the appropriate quantities. |
In our model a phospholipid molecule is represented by a pair of nearest-neighbor acyl chains, linked covalently to each other. |
In the first system, the phospholipid molecules were solvated by 6560 SPC water molecules. |
In phospholipid membranes these loosely associated water molecules are in fast exchange with the bulk water phase. |
The second step involves binding of phospholipid molecules in the active site of the adsorbed enzyme. |