| The filtrate was discarded and the recovered retentate was divided into two equal aliquots. |
| We switched to individual aliquots of buffer solution, prepared in single-use tubes. |
| Mix the diphenylamine reagent, as above, and split it into two aliquots before adding the acetaldehyde. |
| The cooled, clear digest was diluted to 20 ml with distilled water, filtered, and aliquots were taken for analyses. |
| Each pool was divided into three aliquots and a sufficient quantity of CPG was added to one aliquot. |
| Sample digestion for Hg analysis was conducted separately, using 5 g aliquots of homogenized samples. |
