How to use sequencing in a sentence

Looking for sentences and phrases with the word sequencing? Here are some examples.

Sentence Examples As far as i know, people usually produce subclones, map them by hybridization and then start shotgun cloning and sequencing many small clones. We performed DNA amplification, purification, sequencing, and fragment assembly. As sequencing projects had grown larger and larger it had become quite impossible and pointless for journals to publish the sequences in print. The genotypes were confirmed by sequencing both sense and antisense strands using forward and reverse primers in separate reactions. He wanted to talk over the layout and sequencing of portraits in the notebook he'd been carrying. The fragments were cloned and DNA sequencing demonstrated that they actually corresponded to cDNA with proper intron splicing. Once DNA sequencing devices become operable by an unskilled individual anyone will be able to find out their own DNA sequence. The proper orientation and the sequence of the cloned oligonucleotides were checked by nucleotide sequencing. Now not all sequencing projects are carried out on such grandiose scales as the genome projects. A subset of the participants agreed to fully explore alternative strategies for shotgun cloning and sequencing the MAC genome. Standard techniques were used for DNA cloning, restriction enzyme digestion, Southern blot analysis, and DNA sequencing. Nowadays, this code might contain DNA sequencing and the genetic map of a complex organism such as the human body. Before sequencing, the amplified regions were purified from the agarose gel using a GeneClean kit. The cycling profile and subsequent purification and automated sequencing followed protocols outlined in Matthee et al. We used it at several steps in our cloning procedure including recovery of the genomic fragments for shotgun cloning and eventual sequencing. This allowed the purification and sequencing of six positive clones containing the previously determined nucleotide sequence. The positions of primers used for PCR amplification and sequencing and the diagnostic PstI site are indicated. They have wanted the benefits to be followed by the actual dismantlement, so sequencing is going to be another key issue. I've found this sequencing only works sometimes, and it teaches students to confuse a chord's inversion with its function. The nucleotide sequences of the constructed plasmids were verified by DNA sequencing. Show More Sentences It's also a very cleverly put together compilation, sequencing the songs for maximum impact rather than chronological accuracy. The switch condition was manipulated by sequencing the order of tasks across each five-trial series. The final verification of the mutation was ascertained by sequencing in both directions. These guys play live beats, as well as sampling and sequencing their own sounds. The project started with Brock writing and sequencing a demo of the song that we were going to perform. The company uses gene sequencing from a virus to rapidly produce a vaccine. To verify the mutation, researchers used the very latest gene sequencing techniques. This gene sequencing approach may be a valuable tool for further dissecting the role of bacteria in human decomposition. They performed gene sequencing on specimens from 27 patients, creating a profile of the possible genetic causes of these diseases. They have the latest genetic sequencing equipment, million-dollar biosafety labs, and real-time satellite link-ups to compare notes. But the poem is mostly static, because the sequencing of lines, despite the abecedarian scheme, feels inadvertently arbitrary. Adding DMSO or betaine, or using higher reaction temperatures, did not improve sequencing quality. The 5 mismatched residues are probably due to errors in peptide sequencing since they are located towards the end of the sequences. He pointed out that our bipartite collaboration on the worm was exceptional among genome sequencing projects in its success and lack of friction. This is mitigable via proper sequencing of restoration projects and the Control Program. Even small improvements in your body sequencing can lead to big improvements in your shotmaking. Interlocks and circuitry are provided to effect safe automatic sequencing of the joggling machine. One hundred thousand unamplified phage were rescued with Exassist helper phage in SOLR cells to generate a library in pSKII for EST sequencing. Also contributing to the album's strength as a complete piece is the deft sequencing. The structure of the palindrome was confirmed by extensive restriction mapping and sequencing. Show More Sentences Costs are still a few orders of magnitude too high for the sequencing of one's own genome to become commonplace. One representative of each gene was subcloned and sequenced using cycle sequencing and an automatic sequencer. At first I thought it might be a problem with sequencing so I tried playing the record out of order. He has been a lightning rod for criticism, despite his many successes, including his role as the prime mover in the rapid sequencing of the human genome. Two microliters of the resulting liquid was used as a sequencing template. They began by sequencing the SEMG2 gene in humans, chimpanzees, pygmy chimpanzees, gorillas, orang-utans, gibbons, macaques, colobus monkeys, and spider monkeys. The integrity of the new constructs was verified by DNA sequencing. Editing is the process of creating meaning horizontally along a timeline from beginning to end, by placing and sequencing images next to each other in a specific order. Gene sequencing showed that it was derived from the porcine transmissible gastroenteritis virus by the loss of a sizeable chunk of RNA from one of its genes. A single clone was then selected for complete bidirectional sequencing. We checked in each of the 72 lineages whether the original mutation had reverted to its ancestral wild-type state by performing consensus population sequencing. Direct sequencing of this fragment showed that it was colinear with the genomic sequence of PMCHL1, indicating that it corresponded to unspliced transcripts of that gene. Then, after tagging the ends with a dye, the partly replicated fragments are dribbled into fine gel-filled capillary tubes inside a sequencing machine. Long-term sequelae include impairment of auditory attention, visual memory, visuomotor speed, sequencing, and problem solving, motor steadiness, reaction time, and dexterity. The accuracy of each mutagenized plasmid was checked by sequencing. All of today's video machines, like video poker, video blackjack, and video keno, operate using the same microprocessor technology and randomized sequencing as slot machines. While the smaller bacterial genomes can be tackled by direct sequencing, larger ones like that of drosophila or humans require the use of genetic markers. Vaccinia virus was confirmed in all four patients, and gene sequencing in three of the cases linked the virus to the ACAM2000 smallpox vaccine. These include using sequencing software to calculate timings, using mathematic formulas and free timing with reference timings. Normal DNA sequencing methods happen after birth, but there are new methods to test paternity while a mother is still pregnant. Show More Sentences As with the two previous recordings, the sequencing of the poems is the same as in the printed volume. They coordinate the sequencing of aircraft in the traffic pattern and direct aircraft on how to safely join and leave the circuit. The recent sequencing of the Neanderthal and Denisovan genomes shows possible admixture. Genetic sequencing indicates orchids may have arisen earlier, 76 to 84 million years ago during the Late Cretaceous. Recent sequencing of Neanderthal and Denisovan genomes shows that some admixture with these populations has occurred. Such whole genome sequencing projects allow for studies on evolutionary processes involved in speciation. Metagenomic sequencing could prove especially useful for diagnosis when the patient is immunocompromised. On November 20, 2009, the consortium published results of its sequencing effort in Science. A public genome sequencing effort of cotton was initiated in 2007 by a consortium of public researchers. Once both diploid genomes are assembled, then research could begin sequencing the actual genomes of cultivated cotton varieties. This public relations effort gave them some recognition for sequencing the cotton genome. For amino acid sequencing, the two major proteins were sequenced by automated Edman degradation with an Applied Biosystem 473 sequenator. The first cat genome, derived from a single Abyssinian cat named Cinnamon, was released in 2007 with relatively low sequencing coverage. This allows for any sequencing strategy to simply distinguish between osmylated and unosmylated bases. Identification and characterization of novel amphioxus microRNAs by Solexa sequencing. All fungi were identified as Ascomycetes based on their ITS region of rRNA using PCR followed by DNA sequencing. Placental and blood cell RNA samples were pretreated with a Ribo-Zero Gold Kit to remove ribosomal RNA before sequencing library preparation. Researchers had previously been able to use the sequencing of these veritable molecular building blocks to reproduce a binary code. Cycle sequencing reactions of products from both RTPCR assays were performed by using the Big Dye terminator kit. The high degree of variability posed problems for the design of PCR and sequencing primers. Show More Sentences We agree that sequencing accuracy in assessing comparative single nucleotide polymorphism data is important. Identification of Cryptococcus gattii by use of L-canavanine glycine bromothymol blue medium and DNA sequencing. Identification of Cryptococcus gattii by use of Lcanavanine glycine bromothymol blue medium and DNA sequencing. Martin F, Tuskan GA, DiFazio SP, Lammers P, Newcombe G, Podila GK Symbiotic sequencing for the Populus mesocosm. A human gut microbial gene catalogue established by metagenomic sequencing. The process control system utilizes a MicroLogic processor for control function timing and sequencing. The service required is for the complete mitochondrial DNA sequencing of 14,000 independent human DNA samples. Exome sequencing identifies somatic mutations of DNA methyltransferase gene DNMT3A in acute monocytic leukemia. It is classified into PCR, sequencing, northern blotting, southern blotting, molecular cloning, and other nucleic acid applications. Soon after, researchers at the Institute finished sequencing the bacterium Mycoplasm genitalium, the smallest known genome. The authors wish to sincerely thank Chromous Biotech Ltd, Bangalore, India, for sequencing the amplicons. The successful PCR amplicons were sent for direct sequencing to the Chromous Biotech Pvt. In some cases, the researchers also conducted whole genome sequencing or transcriptome sequencing. Transcriptomes are less expensive than sequencing an entire genome and have been developed for other insect pests. Subtractive sequencing allows detection of millions of different RNA molecules in a single specimen. Cadence contrast pulse sequencing technology option to effectively separate the display of contrast agent signals from tissue signals. Libraries were validated on the Bioanalyzer using the High Sensitivity DNA Chip and equimolarly pooled for the sequencing run. In common with most mutation-scanning methods, including Sanger sequencing, CSCE typically will not detect large deletions or duplications. Increasing use of DNA sequencing technology has brought to light a greater understanding of genetic diversity in species. What's more, Celera is sequencing the genomes of six men and women of differing ethnic backgrounds to find single nucleotide polymorphisms. Show More Sentences Diether Lambrechts through whole genome sequencing of colon and endometrial cancers with MMR deficiency. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. Analysis of a marine picoplankton community by 16S rRNA gene cloning and sequencing. Genomic sequencing reveals that the Halobacterium genome contains genes that are homologous to both eukaryotic and bacterial repair genes. In the case of Dryandra, DNA sequencing has revealed such similarity with Banksia that Dryandra are now being reclassified into this genus. Genetic variants within the genus Echinococcus identified by mitochondrial DNA sequencing. For the analysis of the COL4A4 gene, all the exons including splicing sites were amplified by PCR and screened by direct sequencing analysis. Kinetics of psychrophilic anaerobic sequencing batch reactor treating flushed dairy manure. The increased accuracy is due to breakthrough sequencing chemistry, including a new DNA polymerase that decreases indel errors. More than 100 companies participate in the global next generation sequencing informatics market. Molecular typing of isolates of Rickettsia rickettsii by use of DNA sequencing of variable intergenic regions. Widespread use of global positioning system guidance has freed pilots from the sequencing associated with ground flagmen. Genome sequencing in microfabricated high-density picolitre reactors. Using DNA sequencing technology housed at the UO's Genomics Core Facility, scientists sequenced the bacterial 16S ribosomal RNA gene isolated from the samples. An aliquoted FOXL2 PCR product was used for bidirectional DNA sequencing. Differentiation of phytoplasmas associated with sugarcane and gramineous weed white leaf disease and sugarcane grassy shoot disease by RFLP and sequencing. Advances in computers now allow the sequencing of planes hours in advance. Techniques such as PCR, DNA microarray, DNA sequencing, mass spectrometry and flow cytometry have driven discoveries that have changed our understanding of the world. Discordant results between fetal karyotyping and non-invasive prenatal testing by maternal plasma sequencing in a case of uniparental disomy 21 due to trisomic rescue. The projects are based on finding suitable mammoth DNA in frozen bodies, sequencing its genome and, if possible, gradually combining the DNA with elephant cells. Show More Sentences The sequencing of the human genome is only the tip of the iceberg. Their findings led to the discovery of the polymerase chain reaction, which can amplify DNA and has enabled genetic sequencing and genetic fingerprinting. The 2 oligonucleotides harbor adaptors corresponding to universal forward and reverse oligonucleotides that can be used for sequencing after amplification. The Agilent-BioNanomatrix collaboration marks another case of a company that has recently invested in uncommercialized technology for DNA sequencing. Over the next two years, the Neandertal sequencing team plans to reconstruct a draft of the 3 billion bases that made up the genome of Neandertals. Whole-genome sequencing, amplicon sequencing, and transcriptome sequencing for marker discovery are then discussed, covering both approach and technology. The sequencing of the eluted fragment was clone at Chromous, Bangalore. Among the cartilaginous fishes, the elephant shark was selected for sequencing because of its compact genome, which is one-third the size of the human genome. On the basis of amino acid or nucleotide sequencing of the polymerase and capsid regions, NoV can be divided into 6 genogroups and several genotypes. Whole exome sequencing is widely used for identifying and determining genetic variants responsible for various diseases such as Miller Syndrome and Alzheimer's disease. Metagenomic sequencing of an in vitro-simulated microbial community. Because a separate fluorescent marker is used for each of the four bases of DNA, a typical sequencing electrophoretogram appears as a series of colored peaks. In vivo assay and sequencing of the hemagglutinin protein cleavage site classified the virus as a North American genetic lineage of low pathogenicity for chickens. It is now able to offer new services, including antibody humanisation to supplement its antibody engineering, antibody expression and hybridoma sequencing services. The Pedant-Pro system is the ideal application for extracting top-quality functional and structural information from high-throughput sequencing data in reasonable time. Sequence analysis and editing for bisulphite genomic sequencing projects. In this technique phages expressing candidate autoantigens are immunoprecipitated with antibodies in the patient sample and identified by high-throughput DNA sequencing. Examples from Classical Literature At Screaming Circuits, way back in the cobwebby section of the building, we have a through-hole part sequencing and insertion machine. Since then, advancements in microfabrication and bioinformatics, combined with higher sequencing throughput, have driven the rapid development of NGS technologies. Find more words! 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Words that rhyme with sequencing What is the plural of sequencing? What is the adverb for sequencing? What is the adjective for sequencing? What is the noun for sequencing? Use our Sentence Generator Nearby Words sequences sequencers sequencer sequence of events sequenced sequence sequent sequential sequentiality sequentially sequester sequestered 10-letter Words Starting With s se seq sequ seque sequen sequenc sequenci sequencin