How to use assay in a sentence

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Sentence Examples The sensitivity of the assay is not great enough to detect the small quantity of pepsinogen known to be present in blood. Creatinine was measured in all urine samples by a colorimetric assay, using an alkaline picrate method. Although this assay is very sensitive for detecting chromosomal loss, it is unable to detect non-disjunction and chromosome gain. The validity of this assay may be questioned since acetaldehyde is a highly volatile and reactive molecule. The assay is similar to the Southern blot except that restriction enzyme digestion and denaturation of the mRNA are not needed. Only the icteric serum from control patients developed a color change at the end of the assay. This assay is a highly quantitative and direct measure of antibody response to a specific antigen. The standard procedure for this assay was to remove the cell culture medium and add fresh media containing the MTS tetrazolium reagent. All 79 patients were seronegative for the HIV antibody by the enzyme-linked immunosorbent assay. The assay is likely to form a useful tool for clinicians involved with the care and management of patients who regularly use tobacco. The transactivation assay needed to sensitively detect changes in transcription factor activity. Therefore, we report the results of an MN assay for the first time in treated and untreated tuberculosis patients. An array validation program represents the foundation of tests required to establish robust assay performance in a multiplexed environment. In a table, show how many nanomoles of nitrophenol were produced in each of your enzyme assay tubes. One ton of ore contains 29, 167 troy ounces and so use of an assay ton makes the math easy. After incubation, the assay solution with roots was filtered and a 1 mL aliquot from each sample was removed to a new tube. The anesthetic sensitivities of transformed and untransformed siblings were measured on the same dispersal assay plates simultaneously. Respiratory specimens were examined with radiometric broth medium cultures and with the AMPLICOR assay for M tuberculosis. These results are in keeping with our analysis of the polymerase and DNA binding assay for this mutant. One test tube with 2 mL assay solution without root tissue was used as a control. Show More Sentences The most common in vitro assay for toxigenicity is the Elek immunodiffusion test. We therefore evaluated an indirect immunofluorescent antibody assay for the diagnosis of melioidosis. This high activity is mainly attributable to the use of 1 M of betaine as a stabilizing cosolute in all preparation and assay media. Enzyme activity was determined by the transferase assay in crude leaf extracts. Gene mutational damage in the liver can be assessed in the transgenic animal mutation assay. Compared with other genotoxicity tests, the comet assay is a cheap and simple method. This technology offers improved assay performance as well as speed and automation in one system. The mitochondria activity of the bone cells after exposure to various preparations of Chinese medicines was determined by colorimetric assay. However, the ratio might be an overestimate since the liquid assay favors fast-growing type II cells. The bank is to set up a separate unit, SBI Gold and Precious Metals, to assay the gold and jewellery brought in as deposits. Therefore, a high-performance liquid chromatography method was developed to assay sulfadimidine. The concentrations of the phospholipids were determined by phosphate assay subsequent to total digestion by perchloric acid. Immunologic or antigenic fibrinogen can be quantitated by radial immunodiffusion or enzyme-linked immunosorbent assay. Serial dilutions of the test solutions with culture medium were prepared before in vitro assay. He is able to assay the tricky transition from youthful to mature Hughes with body language and vocal modulation. In some cases intercalative ability has been confirmed with the ethidium displacement assay or viscometry. We assay variation in a natural population to assess whether microsatellite markers can be informative for future studies in this species. When the material is then sieved and the finer fraction sent to the assay furnace the gold particles could stay on the screen and be left out. Pools of all sizes tested gave positive signals indicating that all the pool sizes are amenable for the PCR assay. For decades, taste researchers have used a chemical called phenylthiocarbamide to assay a person's capability to sense a bitter taste. Show More Sentences The publication of a reliable and practical assay for thyroid stimulating hormone was a landmark. The fifth passage of cells was used for the migration assay. But what about off-label use of an assay or use of a home brew? In bancroftian filariasis, detection of circulating antigen by enzyme linked immunosorbent assay or rapid immunochromatographic testing has replaced microscopy. In Nicaragua, for example, the national reference laboratory of the Ministry of Health adapted an enzyme linked immunosorbent assay kit for diagnosing dengue fever. In an in vitro assay system using a recombinant SRK protein, the authors observed that SRK protein could interact with several phosphoproteins extracted from stigma cells. But the next afternoon, and perhaps the next, we would return to the same cruxes, and often assay the same options. The plates were then overlaid with an alkaline phosphatase assay solution. The assay was based on the incorporation of digoxigenin labeled dUTP and a biotin labeled primer specific for Shiga toxin genes during PCR amplification. The negative response observed in this assay is consistent with the theory that tumours observed in mouse oncogenicity studies are non-genotoxic in origin. One good quality assurance indicator for the Digene HC2 assay would be to monitor the frequency of oncogenic HPV types in atypical squamous cell specimens. The samples were assayed for CHH by an enzyme-linked immunosorbent assay. The addition of regulatory proteins, troponin and tropomyosin, is known to increase the sliding speed of thin filaments in the in vitro motility assay. It had become a laboratory standard or reference strain for raising antibodies and for challenge in virus neutralization test to detect and assay antibody in serum. Often times, laboratories that perform drug screening assays are unaware of the various adulterants, detection methods, or their effects on the assay. The polymerase chain reaction assay, branched DNA assay and nucleic acid sequence-based amplification assay quantitate human immunodeficiency virus RNA levels. The EBV genome and clonality were detected by Southern blot and PCR assay. This assay is performed in our lab to identify mono and di-sugars in the urine of patients suspected of having galactosuria, lactosuria, fructosuria, etc. Four times over this time period aliquots of cells were sampled to assay. A further 255 longitudinal strike length trench samples and 481 costean samples pending assay and compilation along with duplicate samples. Show More Sentences Sira Technologies has held the patent on a solid-phase immunobead assay that has been used successfully in detecting ciguatoxin and okadaic acid. Trypsin inhibitor assay in both ELIZA test and cuvet was done with using Na-benzoyl-L-arginine-4-nitroanilide as a substrate. The cytotoxicity assay measures the production of Toxin B and the cytopathologic effect of a stool-sample preparation on cultured cells. Cytosine deaminase from the five Aspergillus species were preincubated at various temperature before assay. Comparison of PCR assay and cytotaxonomy for identification of Anopheles fluviatilis sibling species. The production of cytotoxin by culture isolates with characteristic morphology was determined with the cytotoxicity assay. Antioxidant activity applying an improved ABTS radical cation decolorising assay. The third study used gibberellin as an assay for a gender influencing pheromone. Sanaria also makes available to the research community a range of parasitological and entomological reagents and assay services. Highly sensitive two-site immunoradiometric assay of parathyrin, and its clinical utility in evaluating patients with hypercalcemia. Restoring point-of-care testing during parathyroidectomy with a newer parathyroid hormone assay. In this study, the repellency of the mint Clinopodium glabellum against Demacentorvariabilis was studied using an in vitro assay. Real-time PCR assay for detection and relative quantification of Liocarcinus depurator larvae from plankton samples. Indeed, assay interferences attributed to traditional Chinese medicines have already been reported in digoxin immunoassays. Kivalliq Energy Corporation today released complete assay results from the first drill program at the recent Dipole discovery. This assay measures the fluorescence of phagocytized fluorescently labeled Vibrio bacteria. I am withal persuaded that it may prove much more easy in the assay than it now seems at distance. The mouse inoculation assay is currently the most sensitive, specific, and commonly used method for detecting botulinal toxin. Fluocerite in the matrass yields water and at the melting point of glass the matrass is attacked at a distance from the assay. A closely related but nonequivalent glatiramoid molecule was used as a control to establish assay sensitivity. Show More Sentences These parameters further support the usefulness of the new heptaplex PCR assay in routine clinical diagnosis and infection control. The manner wherewith our Lawes assay to moderate the foolish and vaine expences of table-cheare and apparell, seemeth contrarie to it's end. Each crude oil has unique molecular characteristics which are revealed by the use of Crude oil assay analysis in petroleum laboratories. One impediment to Boulton's work was the lack of an assay office in Birmingham. Bituminous coal must meet a set of criteria for use as coking coal, determined by particular coal assay techniques. The clinical world has assumed that the A1C assay reflects average glycemia over the preceding few months. Comparison of the direct radiolabeled antiglobulin assay and the direct immunobead binding test for detection of sperm-associated antibodies. Detection of attograms of antigen by a high-sensitivity enzyme-linked immunoabsorbent assay using a fluorogenic substrate. We evaluated the validity of the assay by analyzing bark beetles intercepted at quarantine stations. Treatment induced cytotoxicity in the cells was determined using the ATP assay. Interpretation and use of the Western blot assay for serodiagnosis of human immunodeficiency virus type-1 infections. Relative binding affinity-serum modified access assay predicts the relative in vivo bioactivity of the xenoestrogens bisphenol A and octylphenol. The assay consists of a colorless substrate and a proenzyme extracted from amoebocyte cells in the blood of horseshoe crabs. Chromogenic Limulus amoebocyte lysate assay for rapid detection of Gram negative bacteriuria. The TR-Ab auto-antibodies were measured in patients with the use of a radioreceptor assay. Serum olanzapine anticholinergic levels, measured by a radioreceptor assay, did not increase significantly from baseline. Hepatic GH binding was determined by radioreceptor assay and hepatic IGF-I mRNA levels were determined by quantitative slot-blot analysis. Evaluation of radiotoxicity after I131 therapy for thyroid cancer using the micronucleus assay. The antithrombin assay uses a chromogenic method and follows similar manufacturer instructions. To address this issue, the MTT assay was performed to assess the Jurkat cell viability upon exposure to cortisol. Show More Sentences This assay can provide earlier detection for clinically significant ketosis and improve ER and CDU throughput. The role of superoxide anion in the autooxidation of epinephrine and simple assay for superoxide dismutase. A spectrophotometric assay for avidin and biotin based on binding of dyes by avidin. Describe the main limitations of the reticulocyte hemoglobin content assay. Advantages of real-time PCR assay for diagnosis and monitoring of canine leishmaniosis. Real-time homogeneous assay of rapid cycle polymerase chain reaction product for identification of Leptonema illini. We report an interference caused by liposomal amphotericin B in the Synchron LX 20 phosphorus assay. The assay is based on a combination of multiplex PCR and biochip array hybridization. Usefulness of enzyme-linked immunosorbent assay using recombinant desmogleins 1 and 3 for serodiagnosis of pemphigus. This observation will facilitate further improvement of the sensitivity of the serodiagnostic test, while maintaining the assay specificity. The cytotoxicity of 22 sesquiterpenoid unsaturated dialdehydes, as determined by the neutral red absorption assay and by protein determination. Each experiment was performed 3 times in duplicates for ICAM-1 assay and 3 times in sextuplicates for viability assay. Random amplified polymorphic DNA for the specific detection of bubaline Echinococcus granulosus by hybridization assay. The microbiologic assay using Lactobacillus casei is believed by some investigators to be a gold standard method for folate measurement. One unit was defined as the amount of protease that released one micromole of p-nitrophenolate per min in the assay conditions. The extent of DNA damage was evaluated by the frequency of micronuclei in cultured peripheral blood lymphocytes and by comet assay. In order to test for structural changes the in vitro micronucleus assay has been widely adopted. Stage-specific adhesion of Leishmania promastigotes to sand fly midguts assessed using an improved comparative binding assay. Real-time quantitative analysis of telomerase activity in breast tumor specimens using a highly specific and sensitive fluorescent-based assay. Immunometric assay of BN 52080, a heptapeptide C-terminal analogue of sorbin. Show More Sentences Extraction of condensed tannins from cervid feed and feces and quantification using a radial diffusion assay. She explains that the new assay measures levels of 11-dehydro thromboxane B2 which allows platelets to clump and which is secreted in the urine. The molecular analysis should include obtaining the CGG repeat number through both polymerase chain reaction assay and Southern blot assay. The authors report a novel liquid chromatography-tandem mass spectrometric assay to replace the RIAs already available. Plaque assay and replication of Tipula iridescent virus in Spodoptera frugiperda ovarian cells. All the technologies used in multiplexing are based on the principle of screening of multiple analytes in single cycle of the assay. Identification of Plasmodium vivax sporozoites in mosquitoes using an enzyme-linked immunosorbent assay. Detection and identification of avian mycoplasmas by polymerase chain reaction and restriction fragment length polymorphism assay. Flow cytometric analysis of natural killer cell function as a clinical assay. The minimum lethal concentration was the lowest dilution at which no motile trichomonads could be observed from an isolate assay. The results of tritiated thymidine assay, colony formation assay, and flow cytometry indicated that Myricanol inhibited the growth of A549 cells. Comparison of MTT colorimetric assay and tritiated thymidine uptake for lymphocyte proliferation assays using chicken splenocytes. To detect calcium concentrations in culture medium, we used a calcium concentration assay kit following the manufacturer's instructions. We assessed the performance of the modified assay, using boiled blood supernatants. The 5' nuclease assay uses fluorogenic allele-specific detection probes that allow PCR amplification and allele detection in a single procedure. In vitro secretion of proteinases and phospholipases was evaluated by plate assay containing BSA and egg yolk respectively. Only selected intervals of the core were split with much of the haematitic breccia material not submitted for assay. The ORAC assay is based largely on the work reported by Glazer's laboratory and depends on the unique properties of phycoerythrins. Detection of picomole levels of hydroperoxides using a fluorescent dichlorofluorescein assay. A follow-up protocol screens single bead eluates from those sublibraries, which demonstrate activity in the assay. Show More Sentences Embolus location affects the sensitivity of a rapid quantitative D-dimer assay in the diagnosis of pulmonary embolism. An assay based on PrASE was set up for screening of the MC1R and CDKN2A polymorphisms suggested to be present in the Swedish population. In Phase 1 clinical trial, Cyt-1010 was well tolerated with no serious adverse effects and was active in the cold pressor assay. Hyaluronidase inhibition assay was studied in vitro using bovine testicular hyaluronidase and human umbilical cord hyaluronic acid. Recommendations have not changed for tests other than the CEA assay, chest x-rays, or flexible proctosigmoidoscopy. To prove the cytotoxic efficacy of P estradiol in Jurkat T-cell model, cell viability was determined by the MTT assay. Analytical performance and clinical utility of a direct LDL-cholesterol assay in a hyperlipidemic pediatric population. The basis of this assay is that cell wall damage effects should be ameliorated under hyperosmotic conditions. Cytokine concentrations were measured in culture supernatants by enzyme-linked immunoadsorbent assay after centrifugation. Briefly, the assay is based on the detection of an immunoreaction between sTfR and sTfR-specific antibodies in liquid phase. The DRG Hepcidin-25 bioactive ELISA Kit is a solid phase enzyme-linked immunosorbent assay based on the competitive principle. Antibodies against ABV were not detected in the plasma of any of the hatched goslings with an enzyme-linked immunosorbent assay. The transparent device is a miniaturised version of the enzymelinked immunosorbent assay test, which is normally bench-top size. A multiplex PCR assay was used with primers used for the K99, Sta, Stx1, Stx2, intimin, and the F41 fimbrial subunit genes. The EAC cells were counted using the Trypan Blue assay to calculate the total viable and inviable cells. The third element involved years of assay standardization, replication of results in multiple laboratories, and comparisons with in vivo results. Novel fluorometric assay for hydroxyl radical prevention capacity using fluorescein as the probe. We assumed that the use of hydrophilic fluorophores would result in fluorophore leakage from the beads and would complicate assay outcomes. Serotype-specific detection of dengue viruses in a fourplex real-time reverse transcriptase PCR assay. Two holes on the Lower Icefall Zone with assay results pending. Show More Sentences One set of triplicates was autoclaved twice, with a 24-hour room temperature incubation between autoclavings, and used as sterile controls for the mineralization assay. Otherwise the GADA fluorometric assay was performed as described above. Here we describe a sensitive and specific adenosine-binding protein assay for detection of adenosine in samples that can be directly applied to deproteinized specimens. The combination of this test, and Bayer's current line of VERSANT HIV and HCV viral nucleic acid tests, delivers on Bayer's strategy to become a leader in NAD assay virology. IgG enzyme immunoassays for MCPyV and TSPyV were conducted as described, except that we omitted subtraction of antigen-free background in the MCPyV assay. John Babish, Paracelsian founder, Vice President of Scientific Affairs and developer of the Assay. Assay results from holes WEL-1 and WEL-2, drilled close to the Shore of Wapawekka Lake, show a copper-zinc-carrying volcanic sulphide system. Assay method for anti-hepatoprotective using galactosamine induced cytotoxicityin primary cultured hepatocytes. Non-isotopic RNase cleavage assay for mutation detection in MEFV, the gene responsible for familial Mediterranean fever, in a cohort of Greek patients. The inhibitory activity was again confirmed with cover slip assay which showed the reduction in adhesion of the cells onto the cover slip surface. The obviation of the label requirement results in a potential simplification of the assay system so that it can also be regarded as self-performing. The novel MaxDiscovery Homocysteine Enzymatic Assay Kit employs simplified endpoint analysis to offer high sensitivity and low detection limits. Each Assay provides clinicians with an easy-to-read patient report and a complimentary psychopharmacologist consultation. Assay method for monitoring the inhibitory effects of antimetabolites on the activity of inosinate dehydrogenase in intact human CEM lymphocytes. A common in vitro assay to assess corrosivity is termed Corrositex. The probe-based, fluorogenic 5' nuclease assay provide excellent sensitivity and the ability to multiplex reactions for real-time quantitation and SNP genotyping. Rapid and reliable genotyping for the Toll-like receptor 4 A896G polymorphism using fluorescence-labeled hybridization probes in a real-time polymerase chain reaction assay. A novel fluorescence resonance energy transfer-based reverse transcriptase polymerase chain reaction assay to simultaneously diagnose and speciate Leishmania spp. Enzyme-linked immunosorbent assay for measurement of IgG antibody to Molluscum contagiosum virus and investigation of the serological relationship of the molecular types. The gnotobiotic assay was performed as described by Lifshitz et al. Show More Sentences Percentages of capacitated and acrosome-reacted spermatozoa were determined by the chlortetracycline fluorescence assay described by Kuroda et al. The indirect haemagglutination assay for hydatid disease was negative. The objective of the assay was to investigate the growth speed and the blood indicators in caimans fed on different diets, considering the different seasons of the year. In current vitamin D assays, blood samples must be prepared and processed to release vitamin from its bound form before it can be measured with an assay. At the end of 20 days, there were data on 22 negative samples and 20 controls at 1024 dilution and 1025 dilution that were used to establish the LOD and LOQ of the assay. Detection of in vivo genotoxicity of haloalkanes and haloalkenes carcinogenic to rodents by the alkaline single cell gel electrophoresis assay in multiple mouse organs. This was proportionally equivalent to the standard reaction mixture for amylase assay based on the enzyme activity on hydrolyzing gelatinized starch. In vivo assay and sequencing of the hemagglutinin protein cleavage site classified the virus as a North American genetic lineage of low pathogenicity for chickens. The dinitrosalicylic reducing sugar assay is commonly used for determination of sugars released from cellulase as well as hemicellulase activity assays. This assay also is known as the gallic acid equivalence method. After the fusion portion of the fire assay process, the resulting lead button is cupelled, oxidizing the lead while leaving a bead of precious metals. Haemolysis, lipaemia and icterus do not interfere with the assay. Radioreceptor assay of haloperidol tissue levels in the rat. Measurement of developmental changes in plasma insulin-like growth factor-I levels of broiler chickens by radioreceptor assay and radioimmunireceptor assay. The IgG anti-HEV assay used in the study was produced with a recombinant peptide of the major HEV structural protein that occurs naturally as a homodimer. The assay results to date show that there is potential to prove up additional resources on the western limb of the Tusker antiform and on the north end of Tusker. We read with interest the recent description of yet another case of bilirubin-related positive interference in an enzymatic colorimetric assay for acetaminophen. The proposed algorithm eliminates the Western blot and recommends the use of an assay that can discriminate between HIV-1 and HIV-2 infection as the second supplemental test. In this research work, chemical composition, phytochemical screening, elemental analysis and anti-oxidant assay of the pulp of Adansonia digitata were carried out. The product then made solely from melted coins would be found wanting when the silversmith took his wares to the Assay Office, thus discouraging the melting of coins. Show More Sentences Venous blood samples of 3 cc were obtained from these children and their HBV markers were tested with the Enzyme-Linked Immunosorbent Assay method. The Modified Prussian Blue Assay is a general screen to actually determine what compounds are present in our unknown plant species Inga Sierrae and Munca Urens. Hyaluronan Binding Assay is originated from the idea of selective mature spermatozoa binding to hyaluronan during the naturel process of fertilization. Examples from Classical Literature If we liken them to gold, she has made her assay and says the gold is pure. Under the conditions of the assay the dioxide cannot be weighed, as it partly dissolves on breaking the current. The ferricyanide had a small influence and, as might be expected, hyposulphite is fatal to the assay. In the following tea assay proper the estimation of theine is not included. For the standard, weigh up 0.5 gram of copper, more or less, according to the quantity judged to be present in the assay. All the places I had to search for her were the stable and the assay office. Even if half an assay ton has been taken the only calculation needed is multiplying the milligrams by two. The method of concentrating and preparing the solution for colorimetric assay is as follows. The manner of working this assay is the same as that adopted in the other colorimetric processes. The Company's assay of the coin, or trial of the pix, a curious proceeding of great solemnity, now takes place every year. To assay bread, the panter kneels, the Carver cuts him a slice, and he eats it. Nevertheless, every assay or panning has a value as indicating the presence of gold. Mr. Kensington, I had this assay made for myself, and not for the public. That which would remain in the cupel if one should assay a phantom. I understand that the assay indicates it is a valuable find. On keeping up the heat, the assay will resume its ordinary train. Mr. daw himself, even as he showed the assay, admired it over and over. Supersensitive time-resolved immunofluorometric assay of free prostate-specific antigen with nanoparticle label technology. But your newsletter says, that an assay was made of the coin. Employed with appropriate reporter genes, this promoter could provide a rapid assay for in vitro testing of chemicals and compositions. Show More Sentences The discrepant thyroid function test results and the absence of clinical symptoms prompted investigations for interference in the TSH assay. Specimens from these mounds, taken from different localities, and at different levels, were sent to San Francisco for more rigid assay and analysis. Summary of results from superimposing additional assay imprecision. Assay of the B-type natriuretic peptide also contributes to standard clinical information in the diagnosis of congestive heart failure. Isolation of antioxidants from Psoralea corylifolia fruits using high-speed counter-current chromatography guided by thin layer chromatography-antioxidant autographic assay. The search ellipse was sub-divided into octants and within each octant the assay data from a maximum of three sample intervals were used for grade estimation. Cytoprint's software was designed to overcome the limitations to assay development where endpoints may not be clearly decipherable using standard imaging algorithms. A small fragment examined under the microscope appeared, from the number of minute entangled air or perhaps steam bubbles, like an assay fused before the blowpipe. Find more words! 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What is the opposite of assay? Words that rhyme with assay What is the past tense of assay? What is the plural of assay? What is the adjective for assay? What is the noun for assay? Translations for assay Use our Sentence Generator Nearby Words assaults assaultive assaulting assaulter assaulted assault assayed assayer assayers assaying assays ass-backwards 5-letter Words Starting With a as ass assa